| 质粒名称: | c-myc promoter (Del 1) |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 16601 |
| 存储实验室: | Bert Vogelstein |
|
载体骨架基本信息 |
|
| 载体名称: | pBV-Luc |
| 载体出品公司: | Vogelstein Lab |
| 空载体大小: | 4867 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳动物表达，荧光素酶 |
| 筛选标记: | -- |
| 载体抗性: | 氨苄霉素,100 μg/mL |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | c-myc promoter (Del 1),MYC |
| 物种来源: | H. sapiens (human) |
| 插入大小: | 2500 bp |
| 基因突变: | Del-1 (XmnI->PvuII) |
| GenBank编号 | -- |
| 启动子: | -- |
| 标签/融合蛋白: | 荧光素酶（主干上的C端） |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | -- |
| 3’克隆位点: | -- |
| 5’测序引物 | -- |
| 3’测序引物 | LucNRev |
|
文献引用方法 |
|
| 材料和方法部分: | c-myc promoter (Del 1) was a gift from Bert Vogelstein (Addgene plasmid # 16601 ; http://n2t.net/addgene:16601 ; RRID:Addgene_16601) |
| 参考文献部分: | Identification of c-MYC as a target of the APC pathway. He TC, Sparks AB, Rago C, Hermeking H, Zawel L, da Costa LT, Morin PJ, Vogelstein B, Kinzler KW. Science. 1998 Sep 4. 281(5382):1509-12. 10.1126/science.281.5382.1509 PubMed 9727977 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
质粒图谱.png)
载体简介
载体序列
LOCUS c-myc promoter (Del 1) 7644 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS c-myc promoter (Del 1)
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7644)
AUTHORS He TC, Sparks AB, Rago C, Hermeking H, Zawel L, da Costa LT, Morin
PJ, Vogelstein B, Kinzler KW
TITLE Identification of c-MYC as a target of the APC pathway.
JOURNAL Science. 1998 Sep 4. 281(5382):1509-12.
PUBMED 9727977
REFERENCE 2 (bases 1 to 7644)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..7644
/organism="synthetic DNA construct"
/mol_type="other DNA"
regulatory 2908..2917
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 2914..4566
/codon_start=1
/gene="luc+"
/product="firefly luciferase"
/label=luciferase
/note="enhanced luc+ version of the luciferase gene"
/translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
primer_bind complement(2980..2998)
/label=LucNrev
/note="Firefly luciferase, reverse primer"
polyA_signal 4607..4728
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(4618..4637)
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind 4672..4691
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind complement(4976..4993)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(5147..5735)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(5227..5246)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(5906..6766)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 6529..6548
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(6767..6871)
/gene="bla"
/label=AmpR promoter
rep_origin 6898..7353
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(6985..7004)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 7195..7216
/label=F1ori-F
/note="F1 origin, forward primer"
polyA_signal 7484..7532
/note="synthetic polyadenylation signal"
misc_feature 7546..7637
/label=pause site
/note="RNA polymerase II transcriptional pause signal from
the human alpha-2 globin gene"
primer_bind 7586..7605
/label=RVprimer3
/note="pGL3 vector, forward primer"
ORIGIN
1 ggtaccgagc tcttacgcgt gctagcccgg gctcgacaga tttcacgttt gccattaccg
61 gttctccata gggtgatgtt cattagcagt ggtgataggt taattttcac catctcttat
121 gcggttgaat agtcacctct gaaccacttt ttcctccagt aactcctctt tcttcggacc
181 ttctgcagcc aacctgaaag aataacaagg aggtggctgg aaacttgttt taaggaaccg
241 cctgtccttc ccccgctgga aaccttgcac ctcggacgct cctgctcctg cccccacctg
301 acccccgccc tcgttgacat ccaggcgcga tgatctctgc tgccagtaga gggcacactt
361 actttacttt cgcaaacctg aacgcgggtg ctgcccagag agggggcgga gggaaagacg
421 ctttgcagca aaatccagca tagcgattgg ttgctccccg cgtttgcggc aaaggcctgg
481 aggcaggagt aatttgcaat ccttaaagct gaattgtgca gtgcatcgga tttggaagct
541 actatattca cttaacactt gaacgctgag ctgcaaactc aacgggtaat aacccatctt
601 gaacagcgta catgctatac acgcacccct ttcccccgaa ttgttttctc ttttggaggt
661 ggtggaggga gagaaaagtt tacttaaaat gcctttgggt gagggaccaa ggatgagaag
721 aatgtttttt gtttttcatg ccgtggaata acacaaaata aaaaatcccg agggaatata
781 cattatatat taaatataga tcatttcagg gagcaaacaa atcatgtgtg gggctgggca
841 actagctaag tcgaagcgta aataaaatgt gaatacacgt ttgcgggtta catacagtgc
901 actttcacta gtattcagaa aaaattgtga gtcagtgaac taggaaatta atgcctggaa
961 ggcagccaaa ttttaattag ctcaagactc cccccccccc aaaaaaaggc acggaagtaa
1021 tactcctctc ctcttctttg atcagaatcg atgcattttt tgtgcatgac cgcatttcca
1081 ataataaaag gggaaagagg acctggaaag gaattaaacg tccggtttgt ccggggagga
1141 aagagttaac ggtttttttc acaagggtct ctgctgactc ccccggctcg gtccacaagc
1201 tctccacttg ccccttttag gaagtccggt cccgcggttc gggtaccccc tgcccctccc
1261 atattctccc gtctagcacc tttgatttct cccaaacccg gcagcccgag actgttgcaa
1321 accggcgcca cagggcgcaa aggggatttg tctcttctga aacctggctg agaaattggg
1381 aactccgtgt gggaggcgtg ggggtgggac ggtggggtac agactggcag agagcaggca
1441 acctccctct cgccctagcc cagctctgga acaggcagac acatctcagg gctaaacaga
1501 cgcctcccgc acggggcccc acggaagcct gagcaggcgg ggcaggaggg gcggtatctg
1561 ctgctttggc agcaaattgg gggactcagt ctgggtggaa ggtatccaat ccagatagct
1621 gtgcatacat aatgcataat acatgactcc ccccaacaaa tgcaatggga gtttattcat
1681 aacgcgctct ccaagtatac gtggcaatgc gttgctgggt tattttaatc attctaggca
1741 tcgttttcct ccttatgcct ctatcattcc tccctatcta cactaacatc ccacgctctg
1801 aacgcgcgcc cattaatacc cttctttcct ccactctccc tgggactctt gatcaaagcg
1861 cggccctttc cccagcctta gcgaggcgcc ctgcagcctg gtacgcgcgt ggcgtggcgg
1921 tgggcgcgca gtgcgttctc ggtgtggagg gcagctgttc cgcctgcgat gatttatact
1981 cacaggacaa ggatgcggtt tgtcaaacag tactgctacg gaggagcagc agagaaaggg
2041 agagggtttg agagggagca aaagaaaatg gtaggcgcgc gtagttaatt catgcggctc
2101 tcttactctg tttacatcct agagctagag tgctcggctg cccggctgag tctcctcccc
2161 accttcccca ccctccccac cctccccata agcgcccctc ccgggttccc aaagcagagg
2221 gcgtggggga aaagaaaaaa gatcctctct cgctaatctc cgcccaccgg ccctttataa
2281 tgcgagggtc tggacggctg aggacccccg agctgtgctg ctcgcggccg ccaccgccgg
2341 gccccggccg tccctggctc ccctcctgcc tcgagaaggg cagggcttct cagaggcttg
2401 gcgggaaaaa gaacggaggg agggatcgcg ctgagtataa aagccggttt tcggggcttt
2461 atctaactcg ctgtagtaat tccagcgaga ggcagaggga gcgagcgggc ggccggctag
2521 ggtggaagag ccgggcgagc agagctgcgc tgcgggcgtc ctgggaaggg agatccggag
2581 cgaatagggg gcttcgcctc tggcccagcc ctcccgctga tcccccagcc agcggtccgc
2641 aacccttgcc gcatccacga aactttgccc atagcagcgg gcgggcactt tgcactggaa
2701 cttacaacac ccgagcaagg acgcgactct cccgacgcgg ggaggctatt ctgcccattt
2761 ggggacactt ccccgccgct gccaggaccc gcttctctga aaggctctcc ttgcagctgg
2821 acgtcgatat cgaattcggg tatataatgg atccggtatc gagatctgcg atctaagtaa
2881 gcttggcatt ccggtactgt tggtaaagcc accatggaag acgccaaaaa cataaagaaa
2941 ggcccggcgc cattctatcc gctggaagat ggaaccgctg gagagcaact gcataaggct
3001 atgaagagat acgccctggt tcctggaaca attgctttta cagatgcaca tatcgaggtg
3061 gacatcactt acgctgagta cttcgaaatg tccgttcggt tggcagaagc tatgaaacga
3121 tatgggctga atacaaatca cagaatcgtc gtatgcagtg aaaactctct tcaattcttt
3181 atgccggtgt tgggcgcgtt atttatcgga gttgcagttg cgcccgcgaa cgacatttat
3241 aatgaacgtg aattgctcaa cagtatgggc atttcgcagc ctaccgtggt gttcgtttcc
3301 aaaaaggggt tgcaaaaaat tttgaacgtg caaaaaaagc tcccaatcat ccaaaaaatt
3361 attatcatgg attctaaaac ggattaccag ggatttcagt cgatgtacac gttcgtcaca
3421 tctcatctac ctcccggttt taatgaatac gattttgtgc cagagtcctt cgatagggac
3481 aagacaattg cactgatcat gaactcctct ggatctactg gtctgcctaa aggtgtcgct
3541 ctgcctcata gaactgcctg cgtgagattc tcgcatgcca gagatcctat ttttggcaat
3601 caaatcattc cggatactgc gattttaagt gttgttccat tccatcacgg ttttggaatg
3661 tttactacac tcggatattt gatatgtgga tttcgagtcg tcttaatgta tagatttgaa
3721 gaagagctgt ttctgaggag ccttcaggat tacaagattc aaagtgcgct gctggtgcca
3781 accctattct ccttcttcgc caaaagcact ctgattgaca aatacgattt atctaattta
3841 cacgaaattg cttctggtgg cgctcccctc tctaaggaag tcggggaagc ggttgccaag
3901 aggttccatc tgccaggtat caggcaagga tatgggctca ctgagactac atcagctatt
3961 ctgattacac ccgaggggga tgataaaccg ggcgcggtcg gtaaagttgt tccatttttt
4021 gaagcgaagg ttgtggatct ggataccggg aaaacgctgg gcgttaatca aagaggcgaa
4081 ctgtgtgtga gaggtcctat gattatgtcc ggttatgtaa acaatccgga agcgaccaac
4141 gccttgattg acaaggatgg atggctacat tctggagaca tagcttactg ggacgaagac
4201 gaacacttct tcatcgttga ccgcctgaag tctctgatta agtacaaagg ctatcaggtg
4261 gctcccgctg aattggaatc catcttgctc caacacccca acatcttcga cgcaggtgtc
4321 gcaggtcttc ccgacgatga cgccggtgaa cttcccgccg ccgttgttgt tttggagcac
4381 ggaaagacga tgacggaaaa agagatcgtg gattacgtcg ccagtcaagt aacaaccgcg
4441 aaaaagttgc gcggaggagt tgtgtttgtg gacgaagtac cgaaaggtct taccggaaaa
4501 ctcgacgcaa gaaaaatcag agagatcctc ataaaggcca agaagggcgg aaagatcgcc
4561 gtgtaattct agagtcgggg cggccggccg cttcgagcag acatgataag atacattgat
4621 gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt
4681 gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat
4741 tgcattcatt ttatgtttca ggttcagggg gaggtgtggg aggtttttta aagcaagtaa
4801 aacctctaca aatgtggtaa aatcgataag gatccgtcga ccgatgccct tgagagcctt
4861 caacccagtc agctccttcc ggtgggcgcg gggcatgact atcgtcgccg cacttatgac
4921 tgtcttcttt atcatgcaac tcgtaggaca ggtgccggca gcgctcttcc gcttcctcgc
4981 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
5041 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
5101 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
5161 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
5221 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
5281 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
5341 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
5401 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
5461 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
5521 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
5581 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
5641 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
5701 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
5761 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
5821 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
5881 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
5941 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
6001 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
6061 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
6121 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
6181 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
6241 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
6301 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
6361 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
6421 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
6481 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
6541 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
6601 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
6661 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
6721 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
6781 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
6841 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
6901 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
6961 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
7021 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
7081 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat
7141 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
7201 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag
7261 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
7321 cgaattttaa caaaatatta acgcttacaa tttgccattc gccattcagg ctgcgcaact
7381 gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagcccaag ctaccatgat
7441 aagtaagtaa tattaaggta cgggaggtac ttggagcggc cgcaataaaa tatctttatt
7501 ttcattacat ctgtgtgttg gttttttgtg tgaatcgata gtactaacat acgctctcca
7561 tcaaaacaaa acgaaacaaa acaaactagc aaaataggct gtccccagtg caagtgcagg
7621 tgccagaaca tttctctatc gata
//
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